Nontargeted metabolomic insights into the behavioral effects of 5-MeO-MiPT in zebrafish (Danio rerio)

Nontargeted metabolomic insights into the behavioral effects of 5-MeO-MiPT in zebrafish (Danio rerio)

Blog Article

5-Methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) is a novel psychoactive substance exhibiting a tryptamine structure.Despite its increasing prevalence, the environmental impact of 5-MeO-MiPT remains unexplored.Our prior investigation revealed that 5-MeO-MiPT induced inhibited spontaneous movement and prompted anxiety-like behavior in adult zebrafish-a validated toxicological model.To elucidate this phenomenon and click here establish a correlation between metabolomics and behavioral changes induced by 5-MeO-MiPT, zebrafish were administered varying drug concentrations.

Zebrafishes were subjected to injections of different 5-MeO-MiPT concentrations.Subsequent metabolomic analysis of endogenous metabolites affected by the drug unveiled substantial variations in metabolic levels between the control group and the drug-injected cohorts.A total of 22 distinct metabolites emerged as potential biomarkers.Further scrutiny identified seven pathways significantly influenced by 5-MeO-MiPT.

A focused exploration into amino acid metabolism, lipid metabolism, and energy metabolism unveiled that the metabolic repercussions of 5-MeO-MiPT on zebrafish resulted in observable brain damage.Notably, the study identified a consequential disruption in the liver-brain read more pathway.The comprehensive metabolomic approach employed herein effectively discerned the impact of 5-MeO-MiPT on zebrafish metabolism.This approach also shed light on the mechanism underpinning the anxiety-like behavior observed in zebrafish post-drug injection.

Specifically, our findings indicate that 5-MeO-MiPT induces brain damage, particularly within the liver-brain pathway.